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chondrogenic differentiation media  (PromoCell)


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    PromoCell chondrogenic differentiation media
    Chondrogenic Differentiation Media, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chondrogenic+differentiation+media/Mesenchymal+Stem+Cell+Chondrogenic+Differentiation+Medium/pm40965742-42-9-13
    Average 95 stars, based on 75 article reviews
    chondrogenic differentiation media - by Bioz Stars, 2026-09
    95/100 stars

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    chondrogenic differentiation media  (Merck & Co)
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    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
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    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
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    hmsc chondrogenic differentiation media  (Cyagen Biosciences)
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    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
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    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
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    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
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    chondrogenic differentiation media  (Thermo Fisher)
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    Thermo Fisher chondrogenic differentiation media
    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
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    stemxvivo chondrogenic differentiation media  (R&D Systems)
    93
    R&D Systems stemxvivo chondrogenic differentiation media
    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for <t>chondrogenic</t> differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II
    Stemxvivo Chondrogenic Differentiation Media, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for chondrogenic differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II

    Journal: Stem Cell Research & Therapy

    Article Title: Immortalized human hair follicle-derived mesenchymal-like stromal cells for the long-term production of scalable Immunomodulatory and regenerative secretome

    doi: 10.1186/s13287-025-04775-8

    Figure Lengend Snippet: iHF-MSC immortalization and phenotyping. A SV40LT mRNA expression by RT-PCR. GAPDH was employed as a reference gene. Positive Control (C+): SV40T plasmid. Negative Control (C-): non-infected HF-MSCs. B Flow cytometry histograms for iHF-MSC surface markers pre-gated on live cells after doublet discrimination. Percentages shown correspond to the proportion of positive cells for the populations indicated in the legend (in order: isotype, HF-MSCs, C18, C20, C26, C39). Histograms shown are representative of one replicate. Y-axis: Count. C iHF-MSC differentiation ability. Optical micrographs of Alizarin Red S staining for osteogenic differentiation, Oil Red O staining for adipogenic differentiation, and Alcian Blue staining for chondrogenic differentiation. D Epifluorescence microscopy images of CD56 immunostaining (DAPI in blue, CD56 in green). Scale bars = 20 μm. Data are representative of 3 independent experiments with 4 replicates per group. Abbreviations: HF-MSCs: hair follicle-derived mesenchymal stromal cells. iHF-MSCs: immortalized HF-MSCs. C18: clone 18. C20: clone 20. C26: clone 26. C39: clone 39. CD: Cluster of Differentiation. MHC-II: major histocompatibility complex class II

    Article Snippet: To do so, cells were seeded at a confluence of 2 × 10 5 cells per well in 96-well U-bottom suspension culture plates and incubated for 24–48 h. Spheroids were then cultured in chondrogenic differentiation media (Merck ready-to-use PromoCell MSC Differentiation Media: C-28012).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Plasmid Preparation, Negative Control, Infection, Flow Cytometry, Staining, Epifluorescence Microscopy, Immunostaining, Derivative Assay, Immunopeptidomics